Smartphone colorimetric assay of acid phosphatase based on a controlled iodine-mediated etching of gold nanorods

Smartphone colorimetric assay of acid phosphatase based on a controlled iodine-mediated etching of gold nanorods
A easy however environment friendly colorimetric assay was developed for the detection and quantification of acid phosphatase (ACP) utilizing a smartphone. This technique relies on target-controlled iodine-mediated etching of gold nanorods (AuNRs).
On account of efficient hydrolysis of the substrate pyrophosphate (PPi) by ACP, chelated Cu2+ with PPi was launched, which promoted the redox response with an iodide ion (I), resulting in the formation of I3. Because the etching agent of AuNRs, I3 triggered a blueshift of the localized floor plasmon resonance peak and, extra importantly, an observable colour change.
The vivid colours have been recorded with a smartphone digital camera and immediately analyzed utilizing an image-processing app. On the idea of the direct correlation between ACP focus and the etching diploma of AuNRs in addition to colour change, this smartphone nanocolorimetry approach confirmed a great linear response towards ACP over the vary of 0-15.Zero U/L, with a detection restrict of 0.97 U/L.
Utilizing the usual addition technique, the sensible applicability of the proposed smartphone-based assay was efficiently demonstrated by figuring out ACP in human serum samples, with outcomes in line with these obtained by UV-Vis spectrophotometry.

Purposeful Evaluation of Mouse G6pc1 Mutations Utilizing a Novel In Situ Assay for Glucose-6-Phosphatase Exercise and the Impact of Mutations in Conserved Human G6PC1/G6PC2 Amino Acids on G6PC2 Protein Expression.

Elevated fasting blood glucose (FBG) has been related to elevated threat for improvement of sort 2 diabetes. Single nucleotide polymorphisms (SNPs) in G6PC2 are an important widespread determinants of variations in FBG in people. Research utilizing G6pc2 knockout mice counsel that G6pc2 regulates the glucose sensitivity of insulin secretion. G6PC2 and the associated G6PC1 and G6PC3 genes encode glucose-6-phosphatase catalytic subunits.
This research describes a practical evaluation of 22 non-synonymous G6PC2 SNPs, that alter amino acids which might be conserved in human G6PC1, mouse G6pc1 and mouse G6pc2, with the objective of figuring out variants that doubtlessly have an effect on G6PC2 exercise/expression. Printed information counsel sturdy conservation of catalytically essential amino acids between all 4 proteins and the associated G6PC3 isoform.
As a result of human G6PC2 has very low glucose-6-phosphatase exercise we used an oblique method, analyzing the impact of those SNPs on mouse G6pc1 exercise.
Utilizing a novel in situ practical assay for glucose-6-phosphatase exercise we display that the amino acid modifications related to the human G6PC2 rs144254880 (Arg79Gln), rs149663725 (Gly114Arg) and rs2232326 (Ser324Professional) SNPs cut back mouse G6pc1 enzyme exercise with out affecting protein expression.
The Arg79Gln variant alters an amino acid mutation of which, in G6PC1, has beforehand been proven to trigger glycogen storage illness sort 1a. We additionally display that the rs368382511 (Gly8Glu), rs138726309 (His177Tyr), rs2232323 (Tyr207Ser) rs374055555 (Arg293Trp), rs2232326 (Ser324Professional), rs137857125 (Professional313Leu) and rs2232327 (Professional340Leu) SNPs confer decreased G6PC2 protein expression.
In abstract, these research determine a number of G6PC2 variants which have the potential to be related to altered FBG in people.

Single laboratory validation of a ready-to-use phosphatase inhibition assay for detection of okadaic acid toxins.

A phosphatase inhibition assay for detection of okadaic acid (OA) toxins in shellfish, OkaTest, was single laboratory validated based on worldwide acknowledged tips (AOAC, EURACHEM). Particular emphasis was positioned on the ruggedness of the tactic and stability of the elements.
All reagents have been secure for greater than 6 months and the tactic was extremely sturdy underneath regular laboratory circumstances. The restrict of detection and quantification have been 44 and 56 µg/kg, respectively; each beneath the European authorized restrict of 160 µg/kg.
The repeatability was evaluated with 2 naturally contaminated samples. The relative customary deviation (RSD) calculated was 1.4% at a stage of 276 µg/kg and three.9% at 124 µg/kg. Intermediate precision was estimated by testing 10 totally different samples (mussel and scallop) on three totally different days and ranged between 2.
Four and 9.5%. The IC(50) values of the phosphatase used on this assay have been decided for OA (1.2 nM), DTX-1 (1.6 nM) and DTX-2 (1.2 nM). The accuracy of the tactic was estimated by restoration testing for OA (mussel, 78-101%; king scallop, 98-114%), DTX-1 (king scallop, 79-102%) and DTX-2 (king scallop, 93%). Lastly, the tactic was qualitatively in comparison with the mouse bioassay and LC-MS/MS.
Smartphone colorimetric assay of acid phosphatase based on a controlled iodine-mediated etching of gold nanorods

New tetrazolium technique for phosphatase assay utilizing ascorbic acid 2-phosphate as a substrate.

A brand new technique to assay alkaline and acid phosphatases assay utilizing ascorbic acid 2-phosphate (AsA-P) and ditetrazolium salt nitroblue tetrazolium chloride (NBT) was developed. AsA-P is hydrolyzed within the presence of phosphatase to yield ascorbic acid. In flip, the ascorbic acid reduces NBT immediately or not directly, opening the tetrazole ring to supply an insoluble formazan as a coloured precipitate.
The proposed technique for alkaline phosphatase was in contrast with a traditional technique by which 5-bromo-4-chloro-3-indolyl phosphate (BCIP) is utilized in mixture with NBT within the dot blots of a dilution collection of beta-lactoglobulin.
AsA-P diminished NBT extra successfully than BCIP within the presence of alkaline phosphatase. AsA-P might be additionally used because the chromogenic substrate for an acid phosphatase assay within the presence of phenazinium methylsulfate and NBT.

A easy colorimetric enzymatic-assay for okadaic acid detection based mostly on the immobilization of protein phosphatase 2A in sol-gel.

Okadaic acid (OA), a lipophilic toxin, is produced by Dinophysis and Prorocentrum, and causes diarrheic shellfish poisoning to people. The mechanism of OA motion relies on the reversible inhibition of protein phosphatase sort 2A (PP2A) by the toxin.
Due to this fact, this inhibition might be used to develop assay for OA detection. On this work, a colorimetric take a look at based mostly on the PP2A inhibition was developed for OA detection. PP2A from GTP and Millipore was immobilized on silica sol-gel, and the detection was carried out.
A restrict of detection of 0.29 and 1.14 μg/L was respectively noticed for enzyme from GTP and Millipore. The immobilization approach supplied a device to protect the enzymatic exercise, which may be very unstable in answer. The PP2A immobilized sol-gel exhibited a storage stability of close to 5 months, when microtiter plate with enzyme-immobilized polymer was saved at -18C°.

QuantiFluo Acid Phosphatase Assay Kit

FACP-100 100
EUR 178
Description: Quantitative fluorimetric (360/450nm) determination of acid phosphatase ACP activity using stable 4-methylumbelliferyl phosphate substrate. Kit size: 100 tests. Detection limit: 0.008 U/L. Shelf life: 6 months. Shipping: RT; storage: -20°C.

Acid Phosphatase Cell Cytotoxicity Assay Kit

K937-500
EUR 370

Acid Phosphatase Activity Fluorometric Assay Kit

K421-500
EUR 349

Acid Phosphatase Activity Colorimetric Assay Kit

K411-500
EUR 359

Alkaline Phosphatase Assay Kit

abx098406-Hitachi7060R190ml2R245ml1 Hitachi 7060; R1: 90ml×2 R2: 45ml×1
EUR 222
  • Shipped within 5-12 working days.

Alkaline Phosphatase Assay Kit

abx098406-Hitachi7170R132ml4R28ml4 Hitachi 7170; R1: 32ml×4 R2: 8ml×4
EUR 222
  • Shipped within 5-12 working days.

Alkaline Phosphatase Assay Kit

abx098406-Toshiba120R150ml4R250ml1 Toshiba 120; R1: 50ml×4 R2: 50ml×1
EUR 222
  • Shipped within 5-12 working days.

Alkaline Phosphatase Assay Kit

abx098406-Toshiba40R150ml4R250ml1 Toshiba 40; R1: 50ml×4 R2: 50ml×1
EUR 222
  • Shipped within 5-12 working days.

Alkaline Phosphatase Assay Kit

abx096002-100Assays 100 Assays
EUR 316
  • Shipped within 5-10 working days.

pNPP Phosphatase Assay Kit

POPN-01K 1000
EUR 340
Description: Quantitative determination of phosphatase activity by colorimetric (405nm) method. Kit size: 1000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C.

pNPP Phosphatase Assay Kit

POPN-500 500
EUR 243
Description: Quantitative determination of phosphatase activity by colorimetric (405nm) method. Kit size: 500 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C.

pNPP Phosphatase Assay Kit

Z5030009 500 assays
EUR 249
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

pNPP Phosphatase Assay Kit

Z5030010 1,000 assays
EUR 249
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Alkaline Phosphatase Assay Kit

Z5030033 250 assays
EUR 660
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.

Sialic Acid Assay Kit

55R-1441 100 assays
EUR 758
Description: Assay Kit for detection of Sialic Acid in the research laboratory

Uric Acid Assay Kit

55R-1451 100 assays
EUR 740
Description: Assay Kit for detection of Uric Acid in the research laboratory

Ascorbic Acid Assay Kit

55R-1489 100 assays
EUR 673
Description: Assay Kit for detection of Ascorbic Acid in the research laboratory

Ascorbic Acid Assay Kit

55R-1491 100 assays
EUR 740
Description: Assay Kit for detection of Ascorbic Acid in the research laboratory

Acid Invertase Assay Kit

abx097992-100Assays 100 Assays
EUR 551
  • Shipped within 5-10 working days.

Sialic Acid Assay Kit

abx098454-Hitachi7060R130ml1R210ml1 Hitachi 7060; R1:30ml×1 R2:10ml×1
EUR 504
  • Shipped within 5-12 working days.

Sialic Acid Assay Kit

abx098454-Hitachi7170R130ml1R210ml1 Hitachi 7170; R1:30ml×1 R2:10ml×1
EUR 504
  • Shipped within 5-12 working days.

Sialic Acid Assay Kit

abx098454-Toshiba40R145ml1R215ml1 Toshiba 40; R1:45ml×1 R2:15ml×1
EUR 676
  • Shipped within 5-12 working days.

Sialic Acid Assay Kit

abx098454-UniversalR145ml1R215ml1 Universal; R1:45ml×1 R2:15ml×1
EUR 676
  • Shipped within 5-12 working days.

Uric Acid Assay Kit

abx098465-Hitachi7020R150ml4R250ml1 Hitachi 7020; R1: 50ml×4 R2: 50ml×1
EUR 222
  • Shipped within 5-12 working days.

Uric Acid Assay Kit

abx098465-Hitachi7060R190ml2R245ml1 Hitachi 7060; R1: 90ml×2 R2: 45ml×1
EUR 222
  • Shipped within 5-12 working days.

Uric Acid Assay Kit

abx098465-UniversalR160ml2R230ml1 Universal; R1: 60ml×2 R2: 30ml×1
EUR 237
  • Shipped within 5-12 working days.

Ascorbic Acid Assay Kit

abx298814-100Assays 100 Assays
EUR 676
  • Shipped within 5-10 working days.

Dehydroascorbic Acid Assay Kit

abx298815-100Assays 100 Assays
EUR 472
  • Shipped within 5-10 working days.

Uric Acid Assay Kit

abx298824-100Assays 100 Assays
EUR 472
  • Shipped within 5-10 working days.

Uric Acid Assay Kit

Z5030021 250 assays
EUR 805
Description: Premade ready to use kits will always come in handy. Get your experiment done right form the first try by using a validated kit with perfectly balanced reagents proportions and compatibility and by following a clear protocol.
The mixture of the simplicity of the colorimetric technique, together with lengthy storage stability achieved by sol-gel immobilization, demonstrated the potentiality of this system for use for business function.

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