A easy however environment friendly colorimetric assay was developed for the detection and quantification of acid phosphatase (ACP) utilizing a smartphone. This technique relies on target-controlled iodine-mediated etching of gold nanorods (AuNRs).

On account of efficient hydrolysis of the substrate pyrophosphate (PPi) by ACP, chelated Cu²⁺ with PPi was launched, which promoted the redox response with an iodide ion (I^–), resulting in the formation of I₃^–. Because the etching agent of AuNRs, I₃^– triggered a blueshift of the localized floor plasmon resonance peak and, extra importantly, an observable colour change.

The vivid colours have been recorded with a smartphone digital camera and immediately analyzed utilizing an image-processing app. On the idea of the direct correlation between ACP focus and the etching diploma of AuNRs in addition to colour change, this smartphone nanocolorimetry approach confirmed a great linear response towards ACP over the vary of 0-15.Zero U/L, with a detection restrict of 0.97 U/L.

Utilizing the usual addition technique, the sensible applicability of the proposed smartphone-based assay was efficiently demonstrated by figuring out ACP in human serum samples, with outcomes in line with these obtained by UV-Vis spectrophotometry.

Elevated fasting blood glucose (FBG) has been related to elevated threat for improvement of sort 2 diabetes. Single nucleotide polymorphisms (SNPs) in G6PC2 are an important widespread determinants of variations in FBG in people. Research utilizing G6pc2 knockout mice counsel that G6pc2 regulates the glucose sensitivity of insulin secretion. G6PC2 and the associated G6PC1 and G6PC3 genes encode glucose-6-phosphatase catalytic subunits.

This research describes a practical evaluation of 22 non-synonymous G6PC2 SNPs, that alter amino acids which might be conserved in human G6PC1, mouse G6pc1 and mouse G6pc2, with the objective of figuring out variants that doubtlessly have an effect on G6PC2 exercise/expression. Printed information counsel sturdy conservation of catalytically essential amino acids between all 4 proteins and the associated G6PC3 isoform.

As a result of human G6PC2 has very low glucose-6-phosphatase exercise we used an oblique method, analyzing the impact of those SNPs on mouse G6pc1 exercise.

Utilizing a novel in situ practical assay for glucose-6-phosphatase exercise we display that the amino acid modifications related to the human G6PC2 rs144254880 (Arg79Gln), rs149663725 (Gly114Arg) and rs2232326 (Ser324Professional) SNPs cut back mouse G6pc1 enzyme exercise with out affecting protein expression.

The Arg79Gln variant alters an amino acid mutation of which, in G6PC1, has beforehand been proven to trigger glycogen storage illness sort 1a. We additionally display that the rs368382511 (Gly8Glu), rs138726309 (His177Tyr), rs2232323 (Tyr207Ser) rs374055555 (Arg293Trp), rs2232326 (Ser324Professional), rs137857125 (Professional313Leu) and rs2232327 (Professional340Leu) SNPs confer decreased G6PC2 protein expression.

In abstract, these research determine a number of G6PC2 variants which have the potential to be related to altered FBG in people.

A phosphatase inhibition assay for detection of okadaic acid (OA) toxins in shellfish, OkaTest, was single laboratory validated based on worldwide acknowledged tips (AOAC, EURACHEM). Particular emphasis was positioned on the ruggedness of the tactic and stability of the elements.

All reagents have been secure for greater than 6 months and the tactic was extremely sturdy underneath regular laboratory circumstances. The restrict of detection and quantification have been 44 and 56 µg/kg, respectively; each beneath the European authorized restrict of 160 µg/kg.

The repeatability was evaluated with 2 naturally contaminated samples. The relative customary deviation (RSD) calculated was 1.4% at a stage of 276 µg/kg and three.9% at 124 µg/kg. Intermediate precision was estimated by testing 10 totally different samples (mussel and scallop) on three totally different days and ranged between 2.

Four and 9.5%. The IC(50) values of the phosphatase used on this assay have been decided for OA (1.2 nM), DTX-1 (1.6 nM) and DTX-2 (1.2 nM). The accuracy of the tactic was estimated by restoration testing for OA (mussel, 78-101%; king scallop, 98-114%), DTX-1 (king scallop, 79-102%) and DTX-2 (king scallop, 93%). Lastly, the tactic was qualitatively in comparison with the mouse bioassay and LC-MS/MS.

A brand new technique to assay alkaline and acid phosphatases assay utilizing ascorbic acid 2-phosphate (AsA-P) and ditetrazolium salt nitroblue tetrazolium chloride (NBT) was developed. AsA-P is hydrolyzed within the presence of phosphatase to yield ascorbic acid. In flip, the ascorbic acid reduces NBT immediately or not directly, opening the tetrazole ring to supply an insoluble formazan as a coloured precipitate.

The proposed technique for alkaline phosphatase was in contrast with a traditional technique by which 5-bromo-4-chloro-3-indolyl phosphate (BCIP) is utilized in mixture with NBT within the dot blots of a dilution collection of beta-lactoglobulin.

AsA-P diminished NBT extra successfully than BCIP within the presence of alkaline phosphatase. AsA-P might be additionally used because the chromogenic substrate for an acid phosphatase assay within the presence of phenazinium methylsulfate and NBT.

Okadaic acid (OA), a lipophilic toxin, is produced by Dinophysis and Prorocentrum, and causes diarrheic shellfish poisoning to people. The mechanism of OA motion relies on the reversible inhibition of protein phosphatase sort 2A (PP2A) by the toxin.

Due to this fact, this inhibition might be used to develop assay for OA detection. On this work, a colorimetric take a look at based mostly on the PP2A inhibition was developed for OA detection. PP2A from GTP and Millipore was immobilized on silica sol-gel, and the detection was carried out.

A restrict of detection of 0.29 and 1.14 μg/L was respectively noticed for enzyme from GTP and Millipore. The immobilization approach supplied a device to protect the enzymatic exercise, which may be very unstable in answer. The PP2A immobilized sol-gel exhibited a storage stability of close to 5 months, when microtiter plate with enzyme-immobilized polymer was saved at -18C°.

pNPP Phosphatase Assay Kit
POPN-01K	BioAssay Systems	1000	EUR 408
Description: Quantitative determination of phosphatase activity by colorimetric (405nm) method. Kit size: 1000 tests. Shelf life: 12 months. Shipping: ambient temp; storage: 4, -20°C.

pNPP Phosphatase Assay Kit
POPN-500	BioAssay Systems	500	EUR 249
Description: For quantitative determination of phosphatase enzyme activity. Key Features: High sensitivity and wide linear range. The detection limit is generally 3 ng phosphatase or below. Homogeneous and simple procedure. No wash or reagent transfer steps are involved. The assay can be completed within 30 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD405nm. Samples: Protein phosphatases. Species: All. Procedure: Assay takes 30 min. Kit size: 500 tests. Detection limit: 3 ng.

pNPP Phosphatase Assay Kit
Z5030009	Biochain	500 assays	EUR 213

pNPP Phosphatase Assay Kit
Z5030010	Biochain	1,000 assays	EUR 213

Alkaline Phosphatase Assay Kit
abx098406-Hitachi7060R190ml2R245ml1	Abbexa	Hitachi 7060; R1: 90ml×2 R2: 45ml×1	EUR 266.4

Alkaline Phosphatase Assay Kit
abx098406-Hitachi7170R132ml4R28ml4	Abbexa	Hitachi 7170; R1: 32ml×4 R2: 8ml×4	EUR 266.4

Alkaline Phosphatase Assay Kit
abx098406-Toshiba120R150ml4R250ml1	Abbexa	Toshiba 120; R1: 50ml×4 R2: 50ml×1	EUR 266.4

Alkaline Phosphatase Assay Kit
abx098406-Toshiba40R150ml4R250ml1	Abbexa	Toshiba 40; R1: 50ml×4 R2: 50ml×1	EUR 266.4

Alkaline Phosphatase Assay Kit
abx096002-100Assays	Abbexa	100 Assays	EUR 379.2

Alkaline Phosphatase Assay Kit
Z5030033	Biochain	250 assays	EUR 651

Human Prostatic Acid Phosphatase AssayLite Antibody (FITC Conjugate)
32921-05141	AssayPro	150 ug	EUR 513.6

Human Prostatic Acid Phosphatase AssayLite Antibody (RPE Conjugate)
32921-05151	AssayPro	150 ug	EUR 513.6

Human Prostatic Acid Phosphatase AssayLite Antibody (APC Conjugate)
32921-05161	AssayPro	150 ug	EUR 513.6

Human Prostatic Acid Phosphatase AssayLite Antibody (PerCP Conjugate)
32921-05171	AssayPro	150 ug	EUR 565.2

QuantiFluo™ Alkaline Phosphatase Assay Kit
QFAP-100	BioAssay Systems	100	EUR 229
Description: For quantitative determination of alkaline phosphatase (ALP) activity using stable 4-methylumbelliferyl phosphate substrate. Key Features: High sensitivity and wide linear range. Use 10 µL sample. Detection limit of 0.02 U/L (20 min reaction). Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 20 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: FL360/450nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 20 min. Kit size: 100 tests. Detection limit: 0.02 U/L.

Alkaline Phosphatase Activity Assay Kit
55R-1402	Fitzgerald	500 assays	EUR 888
Description: Assay Kit for detection of Alkaline Phosphatase in the research laboratory

Alkaline Phosphatase Activity Assay Kit
55R-1406	Fitzgerald	500 assays	EUR 525.6
Description: Assay Kit for detection of Alkaline Phosphatase in the research laboratory

QuantiChrom™ Alkaline Phosphatase Assay Kit
DALP-250	BioAssay Systems	250	EUR 369
Description: For quantitative determination of alkaline phosphatase (ALP) activity using stable p-nitrophenol phosphate substrate. Key Features: High sensitivity and wide linear range. Use 5 µL serum or plasma sample. The detection limit is 2 U/L, linear up to 800 U/L. Homogeneous and simple procedure. Simple "mix-and-measure" procedure allows reliable quantitation of ALP activity within 5 minutes. Robust and amenable to HTS. All reagents are compatible with high-throughput liquid handling instruments. Method: OD405nm. Samples: Serum, plasma etc. Species: All. Procedure: Assay takes 4 min. Kit size: 250 tests. Detection limit: 2 U/L.

Human Acid Phosphatase 1 (ACP1) AssayLite Antibody (RPE Conjugate)
30131-05151	AssayPro	150 ug	EUR 513.6

Human Acid Phosphatase 1 (ACP1) AssayLite Antibody (APC Conjugate)
30131-05161	AssayPro	150 ug	EUR 513.6

Human Acid Phosphatase 1 (ACP1) AssayLite Antibody (FITC Conjugate)
30131-05141	AssayPro	150 ug	EUR 513.6

Human Acid Phosphatase 1 (ACP1) AssayLite Antibody (PerCP Conjugate)
30131-05171	AssayPro	150 ug	EUR 565.2

Acid Phosphatase protein
30-1028	Fitzgerald	1 kU	EUR 619.2
Description: Purified native Acid Phosphatase protein

Acid Phosphatase antibody
20C-CR6098RP	Fitzgerald	100 ug	EUR 165.6
Description: Rabbit polyclonal Acid Phosphatase antibody

Acid Phosphatase Antibody
DF14023	Affbiotech	100ul	EUR 420

Human Prostatic Acid Phosphatase (ACPP) AssayLite Antibody (FITC Conjugate)
35144-05141	AssayPro	150 ug	EUR 513.6

Human Prostatic Acid Phosphatase (ACPP) AssayLite Antibody (RPE Conjugate)
35144-05151	AssayPro	150 ug	EUR 513.6

Human Prostatic Acid Phosphatase (ACPP) AssayLite Antibody (APC Conjugate)
35144-05161	AssayPro	150 ug	EUR 513.6

Acid Phosphatase (Recombinant)
20-abx072013	Abbexa	EUR 1095.60 EUR 5721.60 EUR 393.60	150 µg 1 mg 20 ug

Human Prostatic Acid Phosphatase (ACPP) AssayLite Antibody (PerCP Conjugate)
35144-05171	AssayPro	150 ug	EUR 565.2

The mixture of the simplicity of the colorimetric technique, together with lengthy storage stability achieved by sol-gel immobilization, demonstrated the potentiality of this system for use for business function.