Screening Antibody Libraries with Colony Assay Using scFv-Alkaline Phosphatase Fusion Proteins

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Screening Antibody Libraries with Colony Assay Using scFv-Alkaline Phosphatase Fusion Proteins

Screening antibody libraries is a crucial step in establishing recombinant monoclonal antibodies. The colony assay can determine optimistic clones with out virtually any false-positives; nonetheless, its antibody library is smaller than these utilized in different recombinant screening strategies corresponding to phage show. Thus, to enhance the effectivity of colony assays, it’s needed to extend library […]